Xu Hướng 10/2022 ❤️ Comparative Molecular Cytogenetic Characterization Of Five Wild Vigna Species (Fabaceae) ❣️ Top View | Raffles.edu.vn

Xu Hướng 10/2022 ❤️ Comparative Molecular Cytogenetic Characterization Of Five Wild Vigna Species (Fabaceae) ❣️ Top View

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Chế Độ Tắm Và Phơi Nắng Cho Chào Mào

Chào Mào Trắng Và Cách Nuôi Chăm Sóc Chào Mào Trắng Sinh Sản

Địa Chỉ Bán Chào Mào Bông Đẹp ?

Chào Mào Không Chịu Thi Đấu Ở Cội Lạ

Ép Chào Mào Lên Lửa Trước Khi Đi Thi

Affiliations

1 Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

2 Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

3 College of Biological and Food Engineering, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

4 College of Chemistry and Material Engineering, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

Free PMC article

Free PMC article

Affiliations

1 Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

2 Key Laboratory of Xiangxi Medicinal Plant and Ethnobotany of Hunan Higher Education, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

3 College of Biological and Food Engineering, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

4 College of Chemistry and Material Engineering, Huaihua University, Huaihua, Hunan, 418008, China Huaihua University Huaihua China.

Abstract

To extend our knowledge on karyotype variation of the genus Vigna Savi, 1824, the chromosomal organization of rRNA genes and fluorochrome banding patterns of five wild Vigna species were studied. Sequential combined PI (propidium iodide) and DAPI (4′,6-diamidino-2-phenylindole) (CPD) staining and fluorescence in situ hybridization (FISH) with 5S and 45S rDNA probes were used to analyze the karyotypes of V. luteola (Jacquin, 1771) Bentham, 1959, V. vexillata (Linnaeus, 1753) A. Richard, 1845, V. minima (Roxburgh, 1832) Ohwi & H. Ohashi, 1969, V. trilobata (Linnaeus, 1753) Verdcourt, 1968, and V. caracalla (Linnaeus, 1753) Verdcourt,1970. For further phylogenetic analysis, genomic in situ hybridization (GISH) with the genomic DNA of V. umbellata (Thunberg, 1794) Ohwi & H.Ohashi, 1969 onto the chromosomes of five wild Vigna species was also performed. Detailed karyotypes were established for the first time using chromosome measurements, fluorochrome bands, and rDNA-FISH signals. All species had chromosome number 2n = 2x = 22, and symmetrical karyotypes that composed of only metacentric or metacentric and submetacentric chromosomes. CPD staining revealed all 45S rDNA sites in the five species analyzed, (peri)centromeric GC-rich heterochromatin in V. luteola, V. trilobata and V. caracalla, interstitial GC-rich and pericentromeric AT-rich heterochromatin in V. caracalla. rDNA-FISH revealed two 5S loci in V. caracalla and one 5S locus in the other four species; one 45S locus in V. luteola and V. caracalla, two 45S loci in V. vexillata and V. trilobata, and five 45S loci in V. minima. The karyotypes of the studied species could be clearly distinguished by the karyotypic parameters, and the patterns of the fluorochrome bands and the rDNA sites, which revealed high interspecific variation among the five species. The V. umbellata genomic DNA probe produced weak signals in all proximal regions of V. luteola and all (peri)centromeric regions of V. trilobata. The combined data demonstrate that distinct genome differentiation has occurred among the five species during evolution. The phylogenetic relationships between the five wild species and related cultivated species of Vigna are discussed based on our psent and pvious molecular cytogenetic data.

Keywords: Vigna species; fluorescence in situ hybridization (FISH); fluorochrome banding; karyotype; ribosomal RNA gene (rDNA).

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